Varalakshmi Avula, S. Sundar. P, Sree Rekha. B, Kalpana. M
Varalakshmi Avula1*, S. Sundar. P2, Sree Rekha. B3, Kalpana. M4
1,3,4Department of Pharmaceutical Analysis, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Krishna (Dt), Andhra Pradesh, India.
2Depatment of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada, Krishna (Dt), Andhra Pradesh, India.
Volume - 12,
Issue - 4,
Year - 2022
Milk contains whole nutrients and is consumed by the majority of population in the form of drinking as well as dietary products. Milk adulteration is one of the most common phenomena, this milk adulteration can be over looked in many countries. it shows serious health hazards leading to fatal diseases. Milk adulterants have been reported globally by adding various instances such as adding water, whey proteins, melamine, urea, detergents, starch hydrogen peroxide, boric acid. This paper presents a detailed review of common milk adulterants as well as different methods such as the chromatographic methods such as HPLC and GC coupled with mass spectrometry to detect the adulterants in milk, and immunological techniques such as ELISA and various DNA based procedures like PCR have also been used to detect the adulterants both qualitatively and quantitatively. This study is organized to be an adulterants-based study instead of a techniques-based one, where qualitative detection for most of the common adulterants are enlisted and quantitative detection methods are limited to a few major adulterants of milk. Apart from regular techniques, recent development in these detection techniques has also been reported. Nowadays milk is being adulterated in more sophisticated ways that demand for cutting- edge research for the detection of adulterants. This review intends to contribute towards the common knowledge base regarding possible milk adulterants and their detection techniques.
Cite this article:
Varalakshmi Avula, S. Sundar. P, Sree Rekha. B, Kalpana. M. Analytical methods to detect the Adulterants in Milk - An Overview. Asian Journal of Research in Pharmaceutical Sciences. 2022; 12(4):272-6. doi: 10.52711/2231-5659.2022.00046
Varalakshmi Avula, S. Sundar. P, Sree Rekha. B, Kalpana. M. Analytical methods to detect the Adulterants in Milk - An Overview. Asian Journal of Research in Pharmaceutical Sciences. 2022; 12(4):272-6. doi: 10.52711/2231-5659.2022.00046 Available on: https://ajpsonline.com/AbstractView.aspx?PID=2022-12-4-2
1. Xin H, Stone R. Tainted milk scandal. Chinese probe unmasks high-tech adulteration with melamine. Science. 2008; 322:1310–1.
2. Kamthania M, Saxena J, Saxena K, Sharma DK. Methods of Detection &Remedial Measures. Int J Engg Tech Res. 2014; 1:15–20.
3. Ellis DI, Brewster VL, Dunn WB, Allwood JW, Golovan AP, Goodacre R. Fingerprinting food: current technologies for the detection of food adulteration and contamination. Chem Soc Rev. 2012; 41(17): 5706–27
4. Parminder Singh, Neeraj Gandhi, Milk Preservatives and Adulterants: Processing, Regulatory and Safety Issues, Food Reviews International, 2015; 31(3): 236-261
7. A. Rahman, M.R. Habib, M.Y. Ali, M.A. Islam, M.H. Rashid, Physico-chemical analysis and detection of adulteration in raw milk collected from Goals of different places of Sadarupazila in Mymensingh district, Res. Agric. Livest. Fish, 2017; 4: 99–106, https://doi.org/10.3329/ralf.v4i2.33721
8. M. Krusa, M. Torre, M.L. Marina, A reversed-phase high-performance liquid chromatographic method for the determination of soya bean proteins in bovine milks, Anal. Chem. 2000; 72: 1814–1818, https://doi.org/10.1021/ac990776m
9. S. Tripathy, A.R. Ghole, K. Deep, S.R. Vanjari, S.G. Singh, A comprehensive approach for milk adulteration detection using inherent bio-physical properties as ‘Universal Markers’: towards a miniaturized adulteration detection platform, Food Chem. 2017; 217: 756–765,
10. J.E. Jablonski, J.C. Moore, J.M. Harnly, Nontargeted detection of adulteration of skim milk powder with foreign proteins using UHPLC–UV, J. Agric. Food Chem. 2014; 62: 5198–5206, https://doi.org/10.1021/jf404924x