Author(s): J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi

Email(s): marysheela_j@ethirajcollege.edu.in , Pugazhendhi.a@gmail.com

DOI: 10.52711/2231-5659.2021.00032   

Address: J. Mary Sheela1*, Ravuri. Durga Bhavani2, A. Pugazhendhi3
1Associate Professor, Department of Microbiology, Ethiraj College for Women, Chennai.
2PG Student, Department of Microbiology, Ethiraj College for Women, Chennai.
3Product Manager, J Mitra & Co. Pvt Ltd., Chennai.
*Corresponding Author

Published In:   Volume - 11,      Issue - 3,     Year - 2021


ABSTRACT:
Infection with carbapenem-resistant Enterobacteriaceae (CRE) or carbapenemase-producing Enterobacteriaceae is emerging as an important challenge in health-care.50 swabs were collected from inanimate origins located in Chennai and the organisms isolated were identified by performing various preliminary tests like gram staining, capsule staining, motility, catalase, oxidase and biochemicals. antibiotic sensitivity testing was done for all the isolates using Ampicillin (AMP), Tetracycline (TE), Nitrofurantoin (NIT), Colistin (CL), Piperacillin (PIT), Noroxin (NX), Cefepime (CPM), Cefeparazone (CPZ), Ceftazidime (CFZ), Ciprofloxacin (CIP), Cotrimazole (COT), Ceftriaxone (CFX), Tigecycline (TGC), Tobramycin (TOB), Gentamicin (GEN), Amikacin (AK) and Imipenem (IMP) drugs and highest resistant against all the gram negative drugs was shown by Klebsiella which was further confirmed by MIC. The isolate was analysed for species by 16S rRNA sequencing which was reported as Klebsiella pneumoniae. The main objective of the present study is to identify the genes responsible for showing MDR and given for PCR reported as NDM, OXA-48 and K2A are the genes. Molecular docking was done by retrieving the gene structures of OXA-48 NDM and K2A of K.pneumoniae from protein data bank with proteins present in the leaf part of Cassia auriculata in patchdock and the binding interactions were interpreted and visualized using pyMOL to give a breakpoint by designing a drug for MDR bacterial strains.


Cite this article:
J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi. Characterization and Insilico Analysis of Cassia auriculata against Multi drug Resistant Klebsiella pneumoniae Isolated from Inanimate Origin. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):199-4. doi: 10.52711/2231-5659.2021.00032

Cite(Electronic):
J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi. Characterization and Insilico Analysis of Cassia auriculata against Multi drug Resistant Klebsiella pneumoniae Isolated from Inanimate Origin. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):199-4. doi: 10.52711/2231-5659.2021.00032   Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-3-4


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