J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi
email@example.com , Pugazhendhi.firstname.lastname@example.org
J. Mary Sheela1*, Ravuri. Durga Bhavani2, A. Pugazhendhi3
1Associate Professor, Department of Microbiology, Ethiraj College for Women, Chennai.
2PG Student, Department of Microbiology, Ethiraj College for Women, Chennai.
3Product Manager, J Mitra & Co. Pvt Ltd., Chennai.
Volume - 11,
Issue - 3,
Year - 2021
Infection with carbapenem-resistant Enterobacteriaceae (CRE) or carbapenemase-producing Enterobacteriaceae is emerging as an important challenge in health-care.50 swabs were collected from inanimate origins located in Chennai and the organisms isolated were identified by performing various preliminary tests like gram staining, capsule staining, motility, catalase, oxidase and biochemicals. antibiotic sensitivity testing was done for all the isolates using Ampicillin (AMP), Tetracycline (TE), Nitrofurantoin (NIT), Colistin (CL), Piperacillin (PIT), Noroxin (NX), Cefepime (CPM), Cefeparazone (CPZ), Ceftazidime (CFZ), Ciprofloxacin (CIP), Cotrimazole (COT), Ceftriaxone (CFX), Tigecycline (TGC), Tobramycin (TOB), Gentamicin (GEN), Amikacin (AK) and Imipenem (IMP) drugs and highest resistant against all the gram negative drugs was shown by Klebsiella which was further confirmed by MIC. The isolate was analysed for species by 16S rRNA sequencing which was reported as Klebsiella pneumoniae. The main objective of the present study is to identify the genes responsible for showing MDR and given for PCR reported as NDM, OXA-48 and K2A are the genes. Molecular docking was done by retrieving the gene structures of OXA-48 NDM and K2A of K.pneumoniae from protein data bank with proteins present in the leaf part of Cassia auriculata in patchdock and the binding interactions were interpreted and visualized using pyMOL to give a breakpoint by designing a drug for MDR bacterial strains.
Cite this article:
J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi. Characterization and Insilico Analysis of Cassia auriculata against Multi drug Resistant Klebsiella pneumoniae Isolated from Inanimate Origin. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):199-4. doi: 10.52711/2231-5659.2021.00032
J. Mary Sheela, Ravuri. Durga Bhavani, A. Pugazhendhi. Characterization and Insilico Analysis of Cassia auriculata against Multi drug Resistant Klebsiella pneumoniae Isolated from Inanimate Origin. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):199-4. doi: 10.52711/2231-5659.2021.00032 Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-3-4
1. Ahmed Abduljabbar Jaloob Ajnaby and Janeen Mohammed Reda Jaber Alhasnawi. 2017. Phenotypic and Molecular Characterization of Multidrug Resistance Klebsiella pneumoniae Isolated from Different Clinical Sources in AI-Najaf Province-Iraq. Book of microbiology.20:217-232.
2. Ahmed Abduljabbar Jaloob Aljanaby, Israa Abduljabbar Jaloob Aljanaby. Profile of Antimicrobial Resistance of Aerobic Pathogenic Bacteria isolated from Different Clinical Infections in Al-Kufa Central Hospital –Iraq During period from 2015 to 2017. Research J. Pharm. and Tech 2017; 10(10):3264-3270. doi: 10.5958/0974-360X.2017.00579.0
3. Antonio Domenech-Sanchez, Santiago Hernandez-Alles, Luis Martinez-Martinez, Vicente J. Beneda, and Sebastian Alberta. 1999. Identification and Characterization of a New porin Gene of Klebsiella pneumoniae: Its Role in β-Lactam Antibiotic Resistance. Journal of Bacteriology, 2726+2732.
4. Abu Hena Md Saiful Karim Chowdhury, Sukumar Nandi, Mahbubur Rahman, A S M Ashanul Karim, Syeda Shanoor Hasina Mamtaz, Nura Nasrin Rowshan Ara, et al. 2016. Comparision Between Phenotypic Confirmatory Test & Double Disc Synergy Test in Detection of Extended Spectrum β-Lactamase Produers Among Gram-Negative Bacilli. Journal of microbiology:P. 3-8.
5. B. Reshmi, P. Gopinath. Detection of blaNDM-1gene for the production of MBL in Clinical Strains of Klebsiella pneumoniae. Research J. Pharm. and Tech 2016; 9(10):1618-1620.
6. Cornelius J. Clancy, Liang Chen, Jae H. Hong, Shaoji Cheng, Binghua Hao, Ryan K. Shields, et al. 2013. Mutations of the OmpK36 Porin Gene and Promoter Impact Responses of Sequence Type 258, KPC-2-Producing Klebsiella pneumoniae Strains to Doripenem and Doripenem-Colistin. Antimicrobial Agents and Chemotherapy. p5258-5265.
7. Frank M. Kaczmarek, Faida Dib-Hajj, Wenchi Shang, and Thomas D. Gootz. 2006. High-Level Carbapenem Resistance in a Klebsiella pneumoniae Clinical Isolate Is Due to the combination of blaACT-1 β-Lactamase Production, Porin OmpK35/36 Inservational Inactivation, and Down-Regulation of the Phosphate Transport. Journal of Antimicrobial Agents and Chemotherapy. p, 3396-3406.
8. Hisham A. Abbas, Ashraf A. Kadry, Ghada H. Shaker, Reham M. Goda. Resistance of Escherichia coli and Klebsiella pneumoniae isolated from different Sources to β-lactam Antibiotics. Research J. Pharm. and Tech. 2017; 10(2): 589-591. doi: 10.5958/0974-360X.2017.00116.0
9. K.R Jeya, M. VeeraPagu. Screening for the Antimicrobial Activity of Lippia nodiflora Leaf Extract Against Selective Bacterial Population. Research J. Pharm. and Tech. 4(11): Nov. 2011; Page 1669-1672.
10. Lena Ahmed Saleh Al-Faqeeh, Rafiuddin Naser, Kagne SR. Determination of antibiotic resistant profiles for bacteria isolated from clinical samples in Aurangabad, India. Research J. Pharm. and Tech. 2020; 13(8):3813-3816.
11. Muhammed Ahmad, Carl Urban, Noriel Mariano, Patricia A. Bradford, Ellen Calcagni, Steven J. Projan, Karen Bush, and James J. Rahal. 1999. Clinical Characteristics and Molecular Epidemiology Associated with Imipenem Resistant Klebsiella pneumoniae. Clinical Infectious Diseases 29:352-5.
12. Mandour S. A. 2016. Epidemiological studies using biotyping, serotyping and bacteriocin typing as marker systems to identify clinical multidrug-resistant klebsiella spp. Journal of Antimicrobial Agents and Chemotherapy. p: (378-386).
13. Mohammed Ahad, Gopinath P. Detection of Carbapenemase Resistance among Clinical Isolates of Klebsiella pneumoniae. Research J. Pharm. and Tech. 2016; 9(10):1585-1587.
14. Neil Woodford, Philip M. Tierno Jr., Katherine Young, Luke Tysall, Marie-France I. Palepou, Elaine Ward, et al. 2004. Outbreak of klebsiella pneumoniae producing a New Carbapenem-Hydrolyzig class A β-lactamase, KPC-3, in a New York Medical center. Society of Biotechnology and Microbiology. p:48(12); 4793-4799.
15. Patricia A. Bradford, Carl Urban, Noriel Mariano, Steven J. Projan, James J. Rahal and Karen Bush. 1997. Imipenem Resistance in Klebsiella pneumoniae Is Associated with the Combination of ACT-1, a Plasmid-Medicated AmpC β-Lactamase, and the Loss of na Outer Membrane Protien. Pakistan Journal of Microbiology. p. 563±569.
16. Patricia A. Bradford, Simona Bratu, Carl Urban, Melissa Visalli, Noriel Mariano, David Landman, et al. 2004. Emergence of Carbapenem-Resistant Klebsiella Species Possessing the Class a Carbapenem-Hydrolyzig KPC-2 and Inhibitor -Resistant TEM-30 β-Lactamases in New York City. Society of Clinical Infectious Diseases and Microbiology. p:39:55-60.
17. Poothakuzhiyil Remya, Mariappan Shanthi, Uma Sekhar. 2018. Occurrence and Characterization of Hyperviscous K1 and K2 Serotype in Klebsiella pneumoniae. J. Lab Physicians. P. 10:283-8.
18. R Adrizian, F Suryaningrat, A Alam and D Setiabudi. 2018. Incidence of multidrug-resistant, extensively drug-resistant and pan-drug-resistant bacteria in children hospitalized at Dr. Hasan Sadikin general hospital. Journal of Chicago. p:10.1088/1755.
19. Roumayne L. Ferreira, of Klebsiella pneumoniae strains producing carbapenemases and increase of resistance to colistin in an Italian teaching hospital from January 2012 to December. Journal of clinical Microbiology. p:2014. 15:244.
20. Sara Banu, Gopinath P. Detection of blactx-m Gene for Esbl Resistance among Clinical Isolates of Klebsiella pneumoniae. Research J. Pharm. and Tech 2016; 9(10):1615-1617.
21. Shireen Rana, Siddesh Basawaraj Sirwar, Vijayaraghavan. Prevalence and Antibiogram of Extended Spectrum beta- Lactamase Producing Klebsiella pneumoniae and Proteus mirabilis in UTI. Research J. Pharm. and Tech. 8(11): Nov., 2015; Page 1465-1468.
22. Sukanth kumar Enmozhi, Vijayalakshmi Ganesan, Manigundan Kaari, Bharathi Selvaraj, Gopikrishnan Venugopal, Jerrine Joseph, Radhakrishnan Manikkam. Anti-infective potential of marine actinobacteria against carbapenem resistant Klebsiella pneumoniae ATCC13882. Research J. Pharm. and Tech. 2020; 13(8):3653-3660.
23. Campanini, Marcia C. A. Brito, Eulalia M. L. da Silva, Calo Cesar de Melo Freire Anderson F. da Cunha and Maria-Cristina da Silva Pranchevicius. 2019. High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and β-Lactmase Encoding Genes in a Brazilian Intensive Care Unit. Frontiers in Microbiology P: 2018.03198.
24. Rashid Iqbal, Nadeem Ikram, Muhammad Shoaib, Muhammad Javaid Asad, Raja Tahir Mehmood, Abida Niazi, et al. 2017. Phenotypic Confirmatory Disc Diffusion Test (PCDDT) Double Disc Syergy Test (DDST), E-Test OS Diagnostic tool for Detection of Extended Spectrum Beta Lactamase (ESBL) Producing Uropthogens. Jornal of Applied Biotechnology & Bioengineering. P: 344-349.
25. Yee-Huang Ku, Chi- Chung Chen, Mei-Feng Lee, Yin- Ching Chuang, Hung-Jen Tang, Wen- Liang Yu. 2016. Comparison of Synergism between Colistin, Fosfomycin and tigecycline against extended-spectrum β-Lactamase-producing Klebsiella pneumoniae isolates or with Carbapenem resistance. Journal of Microbiology, P:50. 931-939.