A new, precise, rapid, accurate RP-HPLC method was developed for the Simultaneous Estimation of Olmesartan and Atorvastatin in tablet dosage form. After optimization the good chromatographic separation was achieved by Isocratic mode with a mixture of Acetonitrile: Phosphate Buffer pH 5.8 in the ratio of 60:40 v/v as the mobile phase with ZODIAC C18 (150 × 4.6 mm I.D) 5µm, column as stationary phase at flow rate of 1 mL/min and detection wavelength of 212 nm. The retention times Olmesartan and Atorvastatin found to be 2.67 3 min and 3.717 min respectively. The linearity of this method was found in the concentration range of 60µg/mL to 140 µg/mL for Olmesartanand 30µg/mL to 70µg/mL for Atorvastatin. The correlation coefficient R2 value is found to be 0.9956for Olmesartan and 0.9973for Atorvastatin. The LOD and LOQ for Olmesartan were found to be 15.935ppm and 48.2864ppm respectively. The LOD and LOQ for Atorvastatin were found to be 12.16ppm and 36.86 ppmrespectively. This method was found to be good percentage recovery Olmesartan and Atorvastatin were found to be 100.67 and 99.54 respectively indicates that the proposed method is highly accurate.
Cite this article:
Ajay Kumar D, R Anusha Naik. Simultaneous Estimation of Olmesartan and Atorvastatin by RP-HPLC in Tablet Dosage Form . Asian J. Res. Pharm. Sci. 2018; 8(2):73-80. doi: 10.5958/2231-5659.2018.00015.2