Author(s):
Vidhya K. Bhusari, Sunil R. Dhaneshwar
Email(s):
vidhyabhusari@gmail.com
DOI:
10.52711/2231-5659.2021.00035 
Address:
Vidhya K. Bhusari1*, Sunil R. Dhaneshwar2
1Department of Pharmaceutical Chemistry, Sinhgad Technical Education Society’s, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune, Maharashtra, India.
2Pro Vice Chancellor, Amity University U.P. Lucknow Campus, Lucknow, India.
*Corresponding Author
Published In:
Volume - 11,
Issue - 3,
Year - 2021
ABSTRACT:
Objective: A simple, sensitive, selective, precise repeatable and stability-indicating high-performance thin layer chromatographic method was developed and validated for Eszopiclone in bulk drug and in formulation. Method: Silica gel 60 F-254, TLC precoated aluminium plates was used as the stationary phase for analyzing Eszopiclone and its degradation products, using mobile phase consisting toluene: ethyl acetate: methanol (6: 4: 2 v/v/v). Result: This mobile phase gave compact spots for Eszopiclone with Rf value of 0.52 ± 0.02. Eszopiclone was exposed to hydrolysis, oxidation, neutral and photolytic conditions for conducting stress degradation study. The peak of Eszopiclone and the degradation product was well resolved from each other with a significantly different Rf value. Densitometric estimation of Eszopiclone was performed at 304nm. A good linear plot was obtained in the concentration range of 150-300ng/spot. The method was validated for precision, accuracy (recovery) and robustness study. The limit of detection (LOD) and limit of quantitation (LOQ) was found to be 130ng/spot and 150ng/spot, respectively. Conclusion: The developed HPTLC method can separate Eszopiclone from its degradation products, hence stability studies can be performed using this method.
Cite this article:
Vidhya K. Bhusari, Sunil R. Dhaneshwar. Development and Validation of a Stability-Indicating HPTLC Method for the Estimation of Eszopiclone in Pharmaceutical Dosage Forms. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):219-3. doi: 10.52711/2231-5659.2021.00035
Cite(Electronic):
Vidhya K. Bhusari, Sunil R. Dhaneshwar. Development and Validation of a Stability-Indicating HPTLC Method for the Estimation of Eszopiclone in Pharmaceutical Dosage Forms. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(3):219-3. doi: 10.52711/2231-5659.2021.00035 Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-3-7
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