Author(s):
Vikram Jadhav, Pritam I Pawara, Harshada Pawara
Email(s):
pritampawara34@gmail.com
DOI:
10.52711/2231-5659.2025.00035 
Address:
Vikram Jadhav2, Pritam I Pawara1, Harshada Pawara3
1R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
2Mini Drugs Testing Laboratory, Office of the ADC (I), Central Drugs Standard Control Organization,
M/s JWR Logistic Pvt ltd 15-45, National Highway 4B, Panvel - JNPT Highway, Village Padeghar, Post - Vahal, Panvel - Maharashtra, India.
3DCS.s A.R.A College of Pharmacy, Nagaon Dhule, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 3,
Year - 2025
ABSTRACT:
An innovative, simple, fast, and sensitive RP-HPLC method was used to identify the medication Nirmatrelvir in formulation of tablets in bulk and internally. Using water as the mobile phase (pH 5.5): 60:40 v/v acetonitrile, a good separation was achieved on HPLC. The process was verified for linearity, accuracy, precision, and specificity. Nirmatrelvir's retention time was found to be 12.3, and the method's linearity was suitable for values ranging from 10 to 60 µg/ml, with a correlation coefficient of 0.999. There were no alternative techniques for a single NRT medication. Although some methods were already in place, they could only be applied to Paxlovid tablets that included both Ritonavir and NRT. When the devised approach was used to in-house tablet formulation, the percentage label claim was determined to be between 99.13 and 100.44%. The lowest limits of quantification and detection for nirmatrelvir were determined to be 0.32 and 0.97 µg/ml, respectively.
Cite this article:
Vikram Jadhav, Pritam I Pawara, Harshada Pawara. Development and Validation of a New RP-HPLC Method for Nirmatrelvir in Tablet Formulations and Bulk Pharmaceutical Applications. Asian Journal of Research in Pharmaceutical Sciences. 2025; 15(3):232-6. doi: 10.52711/2231-5659.2025.00035
Cite(Electronic):
Vikram Jadhav, Pritam I Pawara, Harshada Pawara. Development and Validation of a New RP-HPLC Method for Nirmatrelvir in Tablet Formulations and Bulk Pharmaceutical Applications. Asian Journal of Research in Pharmaceutical Sciences. 2025; 15(3):232-6. doi: 10.52711/2231-5659.2025.00035 Available on: https://ajpsonline.com/AbstractView.aspx?PID=2025-15-3-2
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