M.A. Zeeshan Hamza, G. Kumaraswamy, Gandla Lalitha, R. Suthakaran
M.A. Zeeshan Hamza2, G. Kumaraswamy1*, Gandla Lalitha3, R. Suthakaran2
1Research Scholar, Pharmaceutical Analysis, Jawaharlal Nehru Technological University Kakinada, Kakinada -533003. A.P.
2Department of Pharmaceutical Analysis and QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad-500097. Telangana.
3Chilkur Balaji College of Pharmacy, Aziz Nagar, Hyderabad, Telangana.
Volume - 4,
Issue - 4,
Year - 2014
The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium tablets. The chromatographic separation was achieved on a Inertsil C18 ODS (4.6 x 250mm, 5µm) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins.The retention time of Dicloxacillin Sodium and Cefpodoxime Proxetil were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.
Cite this article:
M.A. Zeeshan Hamza, G. Kumaraswamy, Gandla Lalitha, R. Suthakaran. Development and Validation of RP-HPLC for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium Tablets. Asian J. Res. Pharm. Sci. 4(4): Oct.-Dec. 2014; Page 155-159.