Author(s): L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, Alok Kumar, K. Suresh

Email(s): satyadna_l@yahoo.co.in

DOI: Not Available

Address: L. Satyanarayana1*, S.V. Naidu2, M. Narasimha Rao2, Alok Kumar1 and K. Suresh1
1Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034.
2Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam - 530003
*Corresponding Author

Published In:   Volume - 1,      Issue - 3,     Year - 2011


ABSTRACT:
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Darunavir in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5µm partical size, with mobile phase consisting of 0.02M Dipotassium hydrogen orthophosphate +0.02M Potassium Dihydrogen orthophosphatein in water and Acetonitrile in the ratio of 40:60 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 265 nm. The retention time was 5.859 min. The detector response was linear in the concentration of 80-240µg/ml. The respective linear regression equation being Y= 44076.08X +726603. The limit of detection and limit of quantification was 0.1µg and 0.3µg/ml respectively. The percentage assay of Darunavir was 98.58%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Darunavir in bulk drug and in its pharmaceutical dosage form.


Cite this article:
L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, Alok Kumar, K. Suresh. The Estimation of Darunavir in Tablet dosage form by RP-HPLC. Asian J. Res. Pharm. Sci. 1(3): July-Sept. 2011; Page 74-76.


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