Author(s): Vrutti Patel, Ravi A. Manek, Devang B. Sheth.

Email(s): vruttipatel181@gmail.com

DOI: 10.5958/2231-5659.2021.00007.2   

Address: Vrutti Patel, Dr Ravi A. Manek, Dr Devang B. Sheth.
Department of Pharmacology, B. K. Mody Government Pharmacy College, Rajkot, Gujarat, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 1,     Year - 2021


ABSTRACT:
Thrombus and embolus formation may partially or totally obstruct blood flow. This may lead life threatening cardiovascular and cerebrovascular disorders. In obsessive conditions; thrombolytic operators are utilized to disintegrate the effectively shaped clusters in the veins in any case, these medications have certain restrictions which cause genuine and some of the time lethal results. Herbal preparations have been used since ancient times for the treatment of several diseases. Herbal drugs possess variety of phytoconstituents. These phytoconstituents are prominent and promising pharmacological agents with good safety profile. The aim of this study was to identify safe and effective thrombolytic agent from herbal origin, in-vitro. An in-vitro thrombolytic model was utilized to check the coagulation lysis impact of methanolic concentrate of Prunus avium L. organic product alongside Streptokinase as a positive control and DMSO as a negative control. Using an in-vitro thrombolytic model, Prunus avium L. different concentration (5µg/ml, 10µg/ml, 25µg/ml, and 50µg/ml) showed 25.6%, 32.5%, 54.6%, and 59.7% clot lysis activity respectively. Although% clot lysis was found higher with all concentration of extract, the statistically significant increase was found with 25µg/ml and 50µg/ml extract with reference to DMSO as negative control (10.6%) Streptokinase (88.7%) as standard. These herbal extracts possess thrombolytic properties that could lyse blood clots in vitro; however, further assessment of in-vivo thrombolytic activity is necessary to explore its therapeutics benefits.


Cite this article:
Vrutti Patel, Ravi A. Manek, Devang B. Sheth. . Evaluation of in-vitro Thrombolytic activity of methanolic extract of Prunus avium L.Asian J. Res. Pharm. Sci. 2021; 11(1):41-44. doi: 10.5958/2231-5659.2021.00007.2

Cite(Electronic):
Vrutti Patel, Ravi A. Manek, Devang B. Sheth. . Evaluation of in-vitro Thrombolytic activity of methanolic extract of Prunus avium L.Asian J. Res. Pharm. Sci. 2021; 11(1):41-44. doi: 10.5958/2231-5659.2021.00007.2   Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-1-7


1.      Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM and Daginawala HF. Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis. BMC Complementary and Alternative Medicine. 2007; 7: 36: 1-6.

2.      Y SS, panigrahi M, C DG and B BD. Evaluation of in vitro thrombolytic activity of phytochemicals in Bacopa monnieri Linn. Journal of Pharmacy Research. 2012; 5(1): 100-101.

3.      Anwar MD, Khan IN, Md. Sarkar MI, Barua S, Kamal ATM, Zahid Hosen SM. THROMBOLYTIC and Cytotoxic Effect of Different Herbal Extracts. IJPSR. 2011; 2(12): 3118-312.1

4.      Kelley DS, Adkins Y and Laugero KD. A Review of the Health Benefits of Cherries. Nutrients. 2018; 10: 368: 1-22.

5.      Demrow HS, Slane PR, Folts JD, Administration of wine and grape juice inhibits in vivo platelet activity and thrombosis in stenosed canine coronary arteries. Circulation. 1995; 91: 1182-88.

6.      Briggs WH, Folts JD, Osman HE, Administration of raw onion inhibits platelet- mediated thrombosis in dogs. Journal of Nutrition. 2001; 131: 2619-2622.

7.      Duric K, Besovic K, Niksic H, Muratovic S, Sofic E. Anticoagulant activity of some Artemisia dracunculus leaf extracts. Bosn J Basic Med Sci. 2015; 15(2): 9-14.

8.      Mansoor A NH. In vitro study of the anticoagulant activity of some plant extracts. Indian journal of applied research. 2007; 3: 120-122.

9.      Kim JH, Lee J, Kang S, Moon H, Chung KH and Kim KR. Antiplatelet and Antithrombotic Effects of the Extract of Lindera obtusiloba Leaves. Biomol Ther. 2016; 24(6): 659-664.

10.   Zhang HY, Zhang HY, Liu H, Yang M, Wei SF. Antithrombotic Activities of Aqueous Extract from Gardenia Jasminoides and Its Main Constituent. Pharm boil. 2013; 51(2): 221-225.

11.   Chowdhury TA, Kama ATMM, Chowdhury KAAC, Jahan A, Hossain MS, Arafatul Mamur, Hasan M, Hossain J. Cytotoxic and thrombolytic activity of methanolic extract of Macaranga denticulata Bark. The Pharma Innovation Journal. 2015; 4(5): 36-39.

12.   Ali MR, Hossain M, Runa JF, Hasanuzzaman M, Islam MM. Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds. Avicenna J Phytomed. 2014; 4 (6): 430-436.

13.   Khan IN, Md, Habib R, Rahman MM, Mannan A, Sarker MS and Hawlader S. Thrombolytic potential of Ocimum sanctum L., Curcuma longa L., Azadirachta indica L. and Anacardium occidentale L. Journal of Basic and Clinical Pharmacy. 2011; ( 2): 125-127.

14.   Prvulovic D, Malencic D, Popovic M, Ljubojevic M, Ognjanov V. Antioxidant Properties of Sweet Cherries (Prunus avium L.) - Role of Phenolic Compounds. World Academy of Science, Engineering and Technology International Journal of Agricultural and Biosystems Engineering.2011; (5): 676-679.

15.   Souza V. R, Pereira P. A. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chemistry.2014; 156: 362–368.

16.   Hussain F, Islam A, Bulbul L, Moghal MR and Hossain MS. Invitro thrombolytic potential of root extracts of four medicinal plants available in Bangladesh. Anc Sci life. 2014; 33(3): 162-164.

17.   Choi JH, Won Kim DW, Park SE, Lee HJ, Kim KM, Kim KJ, Kim MK, Kim SJ, and Seung Kim S. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille. Journal of Bioscience and Bioengineering. 2014; (20): 1-6.

18.   Bhattacharjee M, kamal R, Roy L. Evaluation of Free Radical Scavenging Activity of ‘Prunus Avium’. Int. J. Pharm. Sci.2016; 38(2): 1-5.

19.   Kelebek H, Selli S. Evaluation of chemical constituents and antioxidant activity of sweet cherry (Prunus avium L.) cultivars. International Journal of Food Science Science and Technology.2011; (46): 2530-2537.

20.   Dar M. A, Tabassum N. Rutin- potent natural thrombolytic agent. International Current Pharmaceutical Journal. 2012; 1(12): 431-435.

Recomonded Articles:

Author(s): K.Vijaya Sri, Guda Santhoshini, D. Ravi Sankar, K.Niharika

DOI: 10.5958/2231-5659.2018.00005.X         Access: Open Access Read More

Author(s): S. S. Shuka, Bina Gidwani, R. Pandey, S. P. Rao, V. Singh, Amber Vyas

DOI:         Access: Open Access Read More

Author(s): Pratibha Gavarkar, Pramod Patil, Akshay Sutar, Shridhar Rasal, Dyaneshwar Surwase

DOI: 10.5958/2231-5659.2016.00029.1         Access: Open Access Read More

Author(s): Sunity Singh, Himanshu Joshi

DOI:         Access: Open Access Read More

Author(s): A. Sowmya, T. Ananthi

DOI:         Access: Open Access Read More

Author(s): A. Elumalai, M. Chinna Eswariah, Vidhyulatha Chowdary CH, Ranjith Kumar, Anusha M, Naresh K.

DOI:         Access: Open Access Read More

Author(s): Debarshi Kar Mahapatra, Ruchi S. Shivhare, Sayan Dutta Gupta

DOI: 10.5958/2231-5659.2018.00006.1         Access: Open Access Read More

Author(s): D.M. Shinkar, Aarif S. Khatik, R.B. Saudagar

DOI: 10.5958/2231-5659.2016.00022.9         Access: Open Access Read More

Author(s): Satheesh Kumar. G, Noorjahan, G. Sadhana Reddy, Syed Khundmeer Mujahid, T. Ashwini, V. Mahender Chary

DOI: 10.5958/2231-5659.2018.00041.3         Access: Open Access Read More

Author(s): Aashwini A. Gholkar, Yogesh P. Nikam, Krushna K. Zambare, Kavya V. Reddy, Akash D. Ghorpade

DOI: 10.5958/2231-5659.2020.00010.7         Access: Open Access Read More

Author(s): Chinedu Fred Anowi, Uyai Umanah, A.U. Emezie, AU Utoh-Nedosa

DOI:         Access: Open Access Read More

Author(s): Yogita Rayate, Shital Shewale, Aishwarya Patil, Manojkumar Nitalikar, Shrinivas Mohite

DOI: 10.5958/2231-5659.2018.00027.9         Access: Open Access Read More

Author(s): Sai Saran Thokada, Harsha Kandregula, Vinodkumar Mugada

DOI: 10.5958/2231-5659.2018.00042.5         Access: Open Access Read More

Author(s): D. D. Rishipathak, A. B. Kokate, K. S. Madhawai

DOI: 10.5958/2231-5659.2017.00032.7         Access: Open Access Read More

Author(s): Srikanth Jupudi, Padmini K., Jaya Preethi P., Deepak Bharadwaj P.V.P., Vengal Rao P.

DOI:         Access: Open Access Read More

Author(s): Keerthi Manukonda, Rama Rao N, Santhosh Aruna M, Lakshmi Prasanna J

DOI:         Access: Open Access Read More

Asian Journal of Research in Pharmaceutical Sciences (AJPSci) is an international, peer-reviewed journal, devoted to pharmaceutical sciences....... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231-5659 


Recent Articles




Tags