Author(s): H. V. Prajapati, D. D. Prajapati, S. A. Bhatt

Email(s): Prajapati.dhaval64@gmail.com

DOI: 10.5958/2231-5659.2021.00006.0   

Address: H. V. Prajapati1, D. D. Prajapati1, S. A. Bhatt2
1Dept. of Microbiology, Mahesana Urban Institute of Science, Ganpat University, Mahesana, India.
2Dept. of Life Science, Hemchandracharya North Gujarat University, Patan, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 1,     Year - 2021


ABSTRACT:
Amylase is the most widely used Food, fermentation, Starch processing, textile and papermaking.This research was conducted to isolate and identify a local amylase producing bacteria from sacred groves soil and characterize, Bacteria from soil were primarily screened on starch agar medium and out of 49 isolates, 15 were found to be amylase producers, The isolates were screened based on their clear zone ratio, enzyme activities The best isolate demonstrated an enzyme activity of MH-31, MH-48 and MH-43, It was then identified by a combination of biochemical tests, morphological and microscopic characteristics along with 16S rRNA gene sequencing from all six isolate the optimization Temp-35°C, pH-7, Carbon source-Glucose, Nitrogen Source-Yeast extract, salt Concentraction: 3 this all condition were recoeded.


Cite this article:
H. V. Prajapati, D. D. Prajapati, S. A. Bhatt. Isolation, Identification, Characterization and Optimization of Amylase producing organisms from sacred groves of Mahesana District. doi: 10.5958/2231-5659.2021.00006.0

Cite(Electronic):
H. V. Prajapati, D. D. Prajapati, S. A. Bhatt. Isolation, Identification, Characterization and Optimization of Amylase producing organisms from sacred groves of Mahesana District. doi: 10.5958/2231-5659.2021.00006.0   Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-1-6


1.      Aqeel, B. M., and Umar, D. M. (2010). Effect of alternative carbon and nitrogen sources on production of alpha-amylase by Bacillus megaterium. World Applied Sciences Journal, 8(13), 85-90.

2.      Bahadure, R. B., Agnihotri, U. S., and Akarte, S. R. (2010). Assay of population density of amylase producing bacteria from different soil samples contaminated with flowing effluents. International Journal of Parasitology Research, 2(1), 9.

3.      Bala, J. D., Abioye, O. P., Auta, H. S., Damisa, D., Adabara, N. U., and Tuggen, T. O. (2013). Screening of soil microorganisms for amylase production. Biotechnology an Indian journal, 22-27.

4.      Ekka, A., and Namdeo, N. (2018). Screening, Isolation and Characterization of Amylase Producing Bacteria and optimization for Production of Amylase. IOSR Journal of Biotechnology and Biochemistry, 4, 50-56

5.      Ekka, A., and Namdeo, N. (2018). Screening, Isolation and Characterization of Amylase Producing Bacteria and optimization for Production of Amylase. IOSR Journal of Biotechnology and Biochemistry, 4, 50-56.

6.      Gupta, A., Gupta, V. K., Modi, D. R., and Yadava, L. P. (2008). Production and characterization of α-amylase from Aspergillus niger. Biotechnology, 7(3), 551-556.

7.      Kandra, L. (2003). α-Amylases of medical and industrial importance. Journal of Molecular Structure: theochem, 666, 487-498.

8.      Luang-In, V., Yotchaisarn, M., Saengha, W., Udomwong, P., Deeseenthum, S., and Maneewan, K. (2019). Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand. Biomedical and Pharmacology Journal, 12(3), 1061-1068.

9.      Luang-In, V., Yotchaisarn, M., Saengha, W., Udomwong, P., Deeseenthum, S., and Maneewan, K. (2019). Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand. Biomedical and Pharmacology Journal, 12(3), 1061-1068.

10.   Lyngwi, N. A., and Joshi, S. R. (2015). ‘Traditional Sacred Groves’, an ethnic strategy for conservation of microbial diversity, Vol. 14(3), 474-480.

11.   Panneerselvam, T., and Elavarasi, S. (2015). Isolation of α-amylase producing Bacillus subtilis from soil. Int. J. Curr. Microbiol. App. Sci, 4(2), 543-552.

12.   Shafiei, M., Ziaee, A. A., and Amoozegar, M. A. (2011). Purification and characterization of an organic-solvent-tolerant halophilic α-amylase from the moderately halophilic Nesterenkonia sp. strain F. Journal of industrial microbiology and biotechnology, 38(2), 275-281.

13.   Shanmugasundaram, S., Annadurai, E., Mayavu, P., Surya, M., and Anbarasu, R. (2015). Screening and Identification of Amylase Producing Bacteria from Marakkanam Saltpan Environment, Tamil Nadu, India. Asian Journal of Biomedical and Pharmaceutical Sciences, 5(48), 35.

14.   Simair, A. A., Qureshi, A. S., Khushk, I., Ali, C. H., Lashari, S., Bhutto, M. A., ... and Lu, C. (2017). Production and partial characterization of α-amylase enzyme from Bacillus sp. BCC 01-50 and potential applications. BioMed research international, 2017.

15.   Van der Maarel, M. J., Van der Veen, B., Uitdehaag, J. C., Leemhuis, H., and Dijkhuizen, L. (2002). Properties and applications of starch-converting enzymes of the α-amylase family. Journal of biotechnology, 94(2), 137-155.

16.   Viswanathan, S., Rohini, S., Rajesh, R., and Poomari, K. (2014). Production and medium optimization of amylase by Bacillus spp using submerged fermentation method. World J Chem, 9(1), 01-06.

17.   Whitehurst RJ, Oort MV. Second Edition. Enzymes in Food Technology. Wiley Blackwell Pub; 2010. p. 44-5.

Recomonded Articles:

Author(s): Mohd. Yaqub Khan, Irfan Aziz, Imtiyaz Ahmad, Bipin Bihari, Hemant Kumar, Manju Panday

DOI:         Access: Open Access Read More

Author(s): Dibyajyoti Saha, Milan Hait, Mayukh Jana, Suprodip Mandal, Utpal Jana

DOI:         Access: Open Access Read More

Author(s): Saudagar R. B., Sarika V. Khandbahale

DOI: 10.5958/2231-5659.2017.00005.4         Access: Open Access Read More

Author(s): Girishchandra R. Mandake, Indrajit S. Patil, Omkar A Patil, Manoj M. Nitalikar, Shriniwas K Mohite

DOI: 10.5958/2231-5659.2018.00012.7         Access: Open Access Read More

Author(s): Nikhil P. Mahalpure , Sheetal B. Gondkar , Ravindra B. Saudagar

DOI: 10.5958/2231-5659.2016.00014.X         Access: Open Access Read More

Author(s): Sana Banu, Syeda Ayesha Farheen

DOI: 10.5958/2231-5659.2019.00015.8         Access: Open Access Read More

Author(s): Rohan R. Vakhariya, Swati S. Talokar, V. R. Salunkhe, C. S. Magdum

DOI: 10.5958/2231-5659.2017.00008.X         Access: Open Access Read More

Author(s): Namdeo G. Shinde, Bhaskar N. Bangar, Sunil M. Deshmukh, Suyog P. Sulake, Dipak P. Sherekar

DOI:         Access: Open Access Read More

Author(s): P. Sairam, Somsubhra Ghosh, Satyabrata Jena, K.N.V Rao, David Banji

DOI:         Access: Open Access Read More

Author(s): Viral Patel

DOI:         Access: Open Access Read More

Author(s): Li Gun, Tian Han, Guo Rui, Du Ning

DOI: 10.5958/2231-5659.2017.00037.6         Access: Open Access Read More

Author(s): J. Kasthuri, A. Cholarajan, R. Vijayakumar, P. Muthukumaran

DOI:         Access: Open Access Read More

Author(s): Smita Kolhe, Praveen Chaudhari, Dhananjay More

DOI:         Access: Open Access Read More

Author(s): Divya B., Sabitha P., Ravindra Reddy, M. Kranthi Kumar Reddy , B.Narasimha Rao

DOI:         Access: Open Access Read More

Author(s): Chinedu Fred Anowi, Uyai Umanah, A.U. Emezie, AU Utoh-Nedosa

DOI:         Access: Open Access Read More

Author(s): Smita S. Aher, Poonam R. Songire, Ravindra B. Saudagar

DOI: 10.5958/2231-5659.2016.00031.X         Access: Open Access Read More

Author(s): Tabasum Siraj Mulla, Monika Sanjay Thorat, Yogita Rayate, Manoj Nitalikar

DOI: 10.5958/2231-5659.2019.00021.3         Access: Open Access Read More

Asian Journal of Research in Pharmaceutical Sciences (AJPSci) is an international, peer-reviewed journal, devoted to pharmaceutical sciences....... Read more >>>

RNI: Not Available                     
DOI: 10.52711/2231-5659 


Recent Articles




Tags