Author(s): Sagar Singh Jough, Rakesh Kumar Saini, Aamna Parveen

Email(s): ssingh99756@gmail.com

DOI: 10.52711/2231-5659.2021-11-2-6   

Address: Sagar Singh Jough1, Rakesh Kumar Saini2, Aamna Parveen3
1Department of Pharmacology, Ch. Sughar Singh Pharmacy College, Etawah, U.P., India.
2Department of Pharmaceutical Chemistry, Ch. Sughar Singh Pharmacy College, Etawah, U.P., India.
3Department of Pharmaceutical Chemistry, Institute of Pharmacy, CSJM University, Kanpur, U.P., India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 2,     Year - 2021


ABSTRACT:
Hypertension is a turning into a significant danger to the world. In the hunt of lead atoms from plant beginning as a substitute for poisonous engineered drugs, 26 Indian restorative plants and nourishments were screened for their ACE (Angiotensin Converting Enzyme) inhibitory movement. IC50 (half restraint of ACE) estimations of hydroalcoholic unrefined concentrates and division were dictated by a colorimetric technique. Dynamic parts were additionally screened to decide the compound energy, mode, explicitness and instrument of restraint. Normalization was finished by deciding aggregate phenolics and flavonoids as gallic corrosive and quercetin counterparts/mg of concentrate individually. Among 26 unrefined concentrates, Cynara scolymus extricate indicated the best action, IC50 esteem 356.62µg/mL. Pro restraint coming about because of protein precipitation was most noteworthy in Coscinium fenestratum. Lineweaver-Burk plots uncovered a serious method of restraint for Punica granatum ethyl acetic acid derivation part. Divisions of Cynara scolymus were seen as vague inhibitors of ACE. Coscinium fenestratum parts restrained the ACE by Zn2+ particle chelation. Further, in the quest for sheltered and powerful lead atoms from normal sources, (MP) Mucuna pruriens L. (Fabaceae) seeds were used for investigating the antihypertensive potential. Generally it is utilized as diuretic and Hypotensive. Bioassay-guided divisions were used for the separation of dynamic mixes by segment chromatography. IC50 esteem, protein energy and restraint system were resolved. In vivo time and portion subordinate hypotensive examination followed by changes in the MAP (Mean blood vessel pressure) actuated by angiotensin I (3 nmol/kg), angiotensin II (3nmol/kg), and to bradykinin (10nmol/kg) in anesthetized rodents was finished. Plasma and tissue ACE exercises were additionally decided. Phytochemical examination by spectroscopic methods uncovered the nearness of realized mixes like genistein, ursolic corrosive and L-DOPA from the ethyl acetic acid derivation and water part separately. In vitro examination uncovered MP ethyl acetic acid derivation portion (MPEA) and genistein as the most dynamic part (IC50 156.45µg/mL) and compound (IC50 253.81µM) individually. Lineweaver-Burk plots uncovered a non-serious method of hindrance. Expert protein precipitation was the recommended instrument for restraint. The concentrate indicated a time and portion subordinate decline in the MAP.


Cite this article:
Sagar Singh Jough, Rakesh Kumar Saini, Aamna Parveen. A Comprehensive Study on Anti-hypertensive properties of Punica granatum (Pomegranate), Cynara scolymus (Artichoke), Coscinium fenestratum (Yellow vine) in Phytopharmacological, Molecular Biology Researches. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(2):126-2. doi: 10.52711/2231-5659.2021-11-2-6

Cite(Electronic):
Sagar Singh Jough, Rakesh Kumar Saini, Aamna Parveen. A Comprehensive Study on Anti-hypertensive properties of Punica granatum (Pomegranate), Cynara scolymus (Artichoke), Coscinium fenestratum (Yellow vine) in Phytopharmacological, Molecular Biology Researches. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(2):126-2. doi: 10.52711/2231-5659.2021-11-2-6   Available on: https://ajpsonline.com/AbstractView.aspx?PID=2021-11-2-6


REFERENCES:
1.    Carlsson, Axel C., et al. "Cardiovascular and total mortality in men and women with different blood pressure levels–A 26-year follow-up." Blood Pressure. 18.3 (2009): 105-110.
2.    Murray, B. A., and R. J. FitzGerald. "Angiotensin converting enzyme inhibitory peptides derived from food proteins: biochemistry, bioactivity and production." Current Pharmaceutical Design. 13.8 (2007): 773-791.
3.    Virani Paras, Virani Kinjal. A Review on New Antihypertensive Agent: Irbesartan. Asian J. Res. Pharm. Sci. 6(1): Jan.-Mar., 2016; Page 34-36.
4.    Vasudevan N J. Flax Seeds – An Overview in Hypertension. Int. J. Adv. Nur. Management. 2017; 5(3): 273-278.
5.    Vasan, Ramachandran S., et al. "Impact of high-normal blood pressure on the risk of cardiovascular disease." New England Journal of Medicine. 345.18 (2001): 1291-1297.
6.    Indu Sharma, Bharat Parashar, Hitesh Kumar Dhamija, Ritu sharma. An Ayurvedic Arena for Hypertension Treatment. Asian J. Pharm. Res. 2(2): April-June 2012; Page 54-58.
7.    Swaty Chapagai, G Rachel Andrews, Nitish Naik. A Study to Assess the Knowledge and Quality of Life of Pacemaker Patients with a view to Develop an Information Booklet. Asian J. Nur. Edu. and Research.2017; 7(1): 108-114.
8.    Garach Bhavikkumar D. The rationale of use of combination therapy in hypertensive patients. Research J. Pharmacology and Pharmacodynamics. 2013; 5(1): 19-26.
9.    Atlas, Steven A., and John H. Laragh. "Atrial natriuretic peptide: a new factor in hormonal control of blood pressure and electrolyte homeostasis." Annual Review of Medicine. 37.1 (1986): 397-414.
10.    Moderation of the Plasma Chemistry and Hematological Indices of Normal and Salt-loaded Rats by an Aqueous Extract of the Rhizomes of Sansevieria Liberica: Implications for the Management of Hypertension and Associated Complications. Asian J. Pharm. Res. 3(3): July-Sept. 2013; Page 134-140.
11.    Esler, Murray. "The sympathetic system and hypertension." American Journal of Hypertension. 13.S4 (2000): 99S-105.
12.    Atlas, Steven A. "The renin-angiotensin aldosterone system: pathophysiological role and pharmacologic inhibition." Journal of Managed Care Pharmacy.  (2007): 9-20.
13.    Brown, Morris J. "Direct renin inhibition—a new way of targeting the renin system." Journal of Renin-Angiotensin-Aldosterone System 7.2 suppl (2006): S7-S11.
14.    Dhananjay Sangle, Amit Naik, Amol Ghorpade, Vikram Ghatkar, Suvarna Ghuge, Dipti Ingle, Rishikesh Ingle. Cost Effectiveness Analysis Study between Atenolol and Amlodipine in Essential Hypertension. Research J. Pharm. and Tech. 6(9): September 2013; Page 1001-1003.
15.    Erdos EG. “The angiotensin I converting enzyme.” Fed Proc. 36.5 (1977): 1760-1765.
16.    Skeggs, Leonard T., Joseph R. Kahn, and Norman P. Shumway. "The preparation and function of the hypertensin-converting enzyme." Journal of Experimental Medicine. 103.3   (1956): 295-299.
17.    Shi, Lijun, et al. "Angiotensin-converting enzymes and drug discovery in cardiovascular diseases." Drug Discovery Today. 15.9 (2010): 332-341.
18.    Carey, Robert M., and Helmy M. Siragy. "Newly recognized components of the reninangiotensin system: potential roles in cardiovascular and renal regulation." Endocrine Reviews. 24.3 (2003): 261-271.
19.    Crackower, Michael A., et al. "Angiotensin-converting enzyme 2 is an essential regulator of heart function." Nature. 417.6891 (2002): 822-828.
20.    Carey, Robert M., and Helmy M. Siragy. "Newly recognized components of the reninangiotensin system: potential roles in cardiovascular and renal regulation." Endocrine Reviews. 24.3 (2003): 261-271.
21.    Ferrario, Carlos M., and Jasmina Varagic. "The ANG-(1–7)/ACE2/mas axis in the regulation of nephron function." American Journal of Physiology-Renal Physiology 298.6 (2010): F1297-F1305.
22.    Verma, Amrisha, et al. "ACE2 and Ang-(1-7) confer protection against development of diabetic retinopathy." Molecular Therapy 20.1 (2012): 28-36.
23.    Paras Virani, Rajanit Sojitra, Bhadresh Savaj, Hasumati Raj, Vineet Jain. Simultaneous Estimation of Irbesartan and Atorvastatin by First Order Derivative Spectroscopic Method in their Synthetic Mixture Use in Hypertension Condition. Asian J. Pharm. Tech. 2015; Vol. 5: Issue 1, Pg 1-7.
24.    Kang, Dae Gill, et al. "Angiotensin converting enzyme inhibitory phenylpropanoid glycosides from Clerodendron trichotomum." Journal of Ethno Pharmacology 89.1 (2003): 151- 154.
25.    HMPC, Assessment report on Cynara scolymus L., folium, in: European Medicines Agency (Ed.) EMA/HMPC/150209/2009, London E14 4HB, United Kingdom, 2011.
26.    Agusta, A., 2003. Coscinium fenestratum (Gaertner) colebr. In lemens, RMHJ and bunyapraphatsara, N. Plant Resour. South East Asia: Med. Poisonous Plants. 3: 139-140.
27.    Facciola S. Cornucopia: a Source Book of Edible Plants. Vista, California: Kampong Publications; 1990. p. 166.
28.    Dewanto, Veronica, Xianzhong Wu, and Rui Hai Liu. "Processed sweet corn has higher antioxidant activity." Journal of Agricultural and Food Chemistry. 50.17 (2002): 4959- 4964.
29.    Diksha Sharma, Dinesh Kumar Mehta, Karun Bhatti, Rina Das, Ram Mohan Chidurala. Amlodipine and Atenolol: Combination Therapy Versus Monotherapy In Reducing Blood Pressure - A Focus On Safety And Efficacy. Research J. Pharm. and Tech. 2020; 13(6): 3007-3013.
30.    Jimsheena, V. K., and Lalitha R. Gowda. "Colorimetric, high-throughput assay for screening angiotensin I-converting enzyme inhibitors." Analytical Chemistry. 81.22 (2009): 9388-9394.
31.    Mohan Krishna Lokireddy, Jayachandra Reddy P, M. Srinivasa Murthy. New Spectrophotometric Method for Assay of Amphotericin B in Bulk and Its Pharmaceutical Formulations. Res. J. Pharm. Dosage Form. and Tech. 6(1): Jan.-Mar. 2014; Page 15-17.
32.    Priyanto, Anugerah Dany, et al. "Screening, discovery, and characterization of angiotensin-Iconverting enzyme inhibitory peptides derived from proteolytic hydrolysate of bitter melon seed proteins." Journal of Proteomics.128 (2015): 424-435.
33.    de Souza, Priscila, et al. "Hypotensive mechanism of the extracts and artemetin isolated from Achillea millefolium L.(Asteraceae) in rats." Phytomedicine. 18.10 (2011): 819-825.
34.    Gallagher, Patricia E., et al. "Estrogen regulation of angiotensin-converting enzyme  mRNA." Hypertension 33.1 (1999): 323-328.
35.    Fujita, Hiroyuki, Tomohide Yamagami, and Kazunori Ohshima. "Effects of an ACEinhibitory agent, katsuobushi oligopeptide, in the spontaneously hypertensive rat and in borderline and mildly hypertensive subjects." Nutrition Research. 21.8 (2001): 1149-1158.

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