Author(s): Pooja A. Patil, Hasumati A. Raj, Gautam B. Sonara

Email(s): patilpooja1611@gmail.com

DOI: 10.5958/2231-5659.2016.00004.7   

Address: Pooja A. Patil1*, Dr. Hasumati A. Raj1, Dr. Gautam B. Sonara2
1Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat.
2Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat.
*Corresponding Author

Published In:   Volume - 6,      Issue - 1,     Year - 2016


ABSTRACT:
It describes Simultaneous estimation of simple, accurate, precise, robust and economical Q-Absorbance ratio spectrophotometric method for Atenolol and Ivabradine HCl in synthetic mixture. Objective: To Delivered information related to Ivabradine HCl and Atenolol combination’s analytical method. Both drugs in combination have not any analytical method done by other or nor published anywhere. So we developed and validated it which is novel method for drugs. Method: Absorbance ratio method for the ratio of absorbance at two selected wavelengths, one which is an iso-absorbtive point and other being the ?-max of one of the two component. Atenolol and Ivabradine HCl show an iso-absorbtive point at 286.40nm in methanol. The second wavelength used is 276nm which is ?-max of Atenolol in methanol. So it is economic in nature. The linearity was obtained in the concentration range of 20-100µg/ml for Atenolol and 2-10µg/ml for Ivabradine HCl. The concentration of the drugs was determined by using ratio of absorbance at iso-absorbtive point and at the ?-max of Atenolol. Result: This method is linear for both drugs; in range 2-10µg/ml for Ivabradine HCl and 20-100µg/ml for Atenolol found at ?max of Atenolol 276nm (R2= 0.9990) and at Iso-absorptive point 286.40nm (R2= 0.9998). % recovery for Ivabradine HCl found 100.47% and Atenolol 100.32%. And all validation parameter (Robustness, Ruggedness, Interday, Intraday) show %RSD >2%. And Limit of detection for Ivabradine HCl and Atenolol at ?1(maximum wavelength) and ?2(Iso-absorptive point) was found 0.309 and 0.181 respectively. % Assay for Ivabradine HCl and Atenolol found to be 100.58% and 100.13% respectively. Conclusion: The method was successfully applied to pharmaceutical synthetic mixture which is considered in approved patent which show no interference. The result of analysis has been validated statistically and by recovery studies. So this method accurate, precise, robust, rugged and economic in nature.


Cite this article:
Pooja A. Patil, Hasumati A. Raj, Gautam B. Sonara. Q-Absorbance Ratio Spectrophotometric method for simultaneous determination of Atenolol and Ivabradine HCl in synthetic mixture. Asian J. Res. Pharm. Sci. 6(1): Jan.-Mar., 2016; Page 27-33. doi: 10.5958/2231-5659.2016.00004.7


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