Author(s):
Ojaswi Ghadge, Supriya Mahajan
Email(s):
ojaswi.ghadge@svbpharmacy.edu.in , drojaswighadge@gmail.com
DOI:
10.52711/2231-5659.2024.00053 
Address:
Ojaswi Ghadge1,2*, Supriya Mahajan1
1C.U. Shah College of Pharmacy, Juhu, Santacruz (W), Mumbai, Maharashtra, India 400049.
2SVB’s College of Pharmacy, Dombivli (E), Maharashtra, India 421204.
*Corresponding Author
Published In:
Volume - 14,
Issue - 4,
Year - 2024
ABSTRACT:
Cancer is still one of the leading causes of death worldwide. Considering the benefits of small molecules for treating malignancies, novel derivatives of benzothiazole were synthesised (1-8). These compounds were subjected to Allium cepa assay for evaluating cytotoxic, antimitotic and genotoxic potential. Cytotoxicity was evaluated by observing root growth inhibition. Antimitotic effect was evaluated by calculating mitotic index (MI). Genotoxicity was studied by observing chromosomal aberrations (CA). The studies were carried out in three different concentrations of tests and controls. As compared to negative control, lesser root growth was observed at all concentration of the test compounds. Among all tested compounds, Compound 1 showed maximum root growth inhibition for all the three concentrations. Dose-dependent inhibitory effect was exhibited on root meristems. During the determination of antimitotic activity, Compound 1 showed least MI value and Compound 3 showed highest MI value. Compounds 1, 2 and 4 exhibited insignificant chromosomal aberrations (CA). Among these, Compound 2 had shown the least per cent chromosomal aberration at all the three concentrations. The statistical analysis was carried out using MAX-STAT-LITE Software, 3.60. The one-way ANOVA at 95% confidence level was used for statistical analysis. Values of p<0.05 were considered to be significant.
Cite this article:
Ojaswi Ghadge, Supriya Mahajan. Evaluation of Synthesised heterocyclic compounds for their Antimitotic activity by Onion Root Tip assay. Asian Journal of Research in Pharmaceutical Sciences. 2024; 14(4):333-8. doi: 10.52711/2231-5659.2024.00053
Cite(Electronic):
Ojaswi Ghadge, Supriya Mahajan. Evaluation of Synthesised heterocyclic compounds for their Antimitotic activity by Onion Root Tip assay. Asian Journal of Research in Pharmaceutical Sciences. 2024; 14(4):333-8. doi: 10.52711/2231-5659.2024.00053 Available on: https://ajpsonline.com/AbstractView.aspx?PID=2024-14-4-1
REFERENCES:
1. World Health Organization: Cancer- Fact sheets, 1st February 2018, http://www.who.int/news-room/fact-sheets/detail/cancer- Retrieved on March 2018.
2. Salimon J., Salih N., Yousif E., Hameed A., Ibraheem H. Synthesis and antibacterial activity of some new 1,3,4-oxadiazole and 1,3,4-thiadiazole derivatives. Australian Journal of Basic Appllied Sciences. 2010; 4(7): 2016–2021.
3. Kamelia M. A., Ossama M. B., Doaa E. R., Usama A., Mohamed M. A. Design, synthesis, anticancer evaluation and molecular docking of V600EBRAF inhibitors derived from pyridopyrazinone. European Journal of Chemistry. 2016; 7(1): 19-29.
4. McKee M. L., Kerwin S. M. Synthesis, metal ion binding, and biological evaluation of new anticancer 2-(2'-hydroxyphenyl) benzoxazole analogs of UK-1. Bioorganic and Medicinal Chemistry. 2008; 16: 1775-1783.
5. Bhat M., Belagali L., Hemanth Kumar K., Mahadeva Kumar S. Synthesis and characterization of novel benzothiazole amide derivatives and screening as possible antimitotic and Antimicrobial Agents. Research on Chemical Intermediates. 2017; 43: 361–378.
6. Irfan A., Batool F., Zahra Naqvi S., Islam A., Osman S., Nocentini A. Benzothiazole derivatives as anticancer agents. Journal of enzyme inhibition and Medicinal Chemistry. 2019; 35: 265-279.
7. Galal A. M., Nabil M., Randa H., Samia A. M. Design, synthesis, docking and evolution of fused Imidazoles as anti-inflammatory and antibacterial agents. Scholars Research Library, Der Pharma Chemica. 2013; 241-255.
8. Padalkar V. S., Borse B. N., Gupta V. D., Phatangare K. R., Patil V. S., Umape P. G., Sekar N. Synthesis and antimicrobial activity of novel 2-substituted benzimidazole, benzoxazole and benzothiazole derivatives. Arabian Journal of Chemistry. 2016; 9(2): S1125-S1130.
9. Gurdal E. E., Buclulgan E., Durmaz I., Cetin R., Yarim M. Synthesis and anticancer activity evaluation of some benzothiazole-piperazine derivatives. Anti-Cancer Agents in Medicinal Chemistry. 2015; 15: 382-389.
10. Konda Ravi Kumar, K.N.S. Karthik, P. Reshma Begum, Ch. M.M. Prasada Rao, Synthesis, characterization and biological evaluation of benzothiazole derivatives as potential antimicrobial and analgesic agents. Asian J. Res. Pharm. Sci. 2017; 7(2): 115-119.
11. Vrushali N Patil, Ameya Yadav, AS Bobade, SV Athlekar, LS Patil, Abhay Chowdhary. Synthesis and Biological Evaluation of New Benzothiazole Derivatives. Asian J. Research Chem. 2009; 2(4): 513-515.
12. Hunasnalkar Shivraj G, Shaikh Gazi, Patil SM, Surwase Ulhas S. Synthesis and Biological Activities of Some Benzothiazole Derivatives. Asian J. Research Chem. 2010; 3(2): 421-427.
13. Gupta Akhilesh, Rawat Swati. Therapeutic Importance of Benzothiazole: Review. Asian J. Research Chem. 2010; 3(4): 821-836
14. Varsha A. Dighe, Rohini R. Pujari. Synthesis of some Sulphur and Nitrogen containing Heterocyclic Compounds. Asian J. Pharm. Res. 2017; 7(1): 21-24.
15. Mahendrasinh M. Raj, Hemul V. Patel, Lata M. Raj, Naynika K. Patel. Novel Heterocyclic Compounds: Synthesis, Characterization and Biological Evaluation. Asian J. Research Chem. 2013; 6(7): 628-633.
16. Ibanrikynti T., Manash P. B., Surya B. P. Inhibitory effect of propolis on onion (Allium cepa) root tip meristem cells. Journal of Science. 2015; 5(12): 1345-1349.
17. Namita K., Sonia S. Allium cepa root chromosomal aberration assay: A review. Indian Journal of Pharmacological and Biological Research. 2013; 1(3): 105-119.
18. Mudeenahally H. K., Basavaiah U., Santhekasalagere B. S. and Yeriyur B. B. Synthesis and study of antimitotic activity of new tetralone esters. International Journal of Chemical and Pharmaceutical Sciences. 2014; 5(3): 105-111.
19. Tulay A.¸ Ozlem S. Evaluation of cytotoxicity and genotoxicity of Inula viscosa leaf extracts with Allium Test. Journal of Biomedicine and Biotechnology. 2010. DOI:10.1155/2010/ 189252.
20. Peter F., Tomaz A. Allium chromosome aberration test for evaluation effect of cleaning municipal water with constructed wetland (CW) in Sveti Tomaz, Sloveni. Journal of Bioremediation & Biodegradation. 2013; 4(4): 189. DOI:10.4172/2155-6199.1000189.
21. Mahesh B., Belagali S., Hemanth K., Mahadeva K. Synthesis and characterization of novel benzothiazole amide derivatives and screening as possible antimitotic and antimicrobial agents. Research on Chemical Intermediates. 2016. DOI 10.1007/s11164-016-2627-3
22. Fiskesjo G. Allium test for screening chemicals; evaluation of cytological parameters-In plants for environmental Studies, Edited by W. Wang, J.W. Gorsuch and J. S. Hughes. CRC Lewis Publishers, New York, 1997; 307-333.
23. Kovalchuck O., Kovalchuck L., Arkhipov A., Telyuk P., Hohn B., Kovalchuck L. The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabitat areas in the Ukraine contaminated by the Chernobyl accident. Mutational Research. 1998; 415: 47–57.
24. Sabti K. Allium test for air and water borne pollution control. Cytobioscience. 1989; 58: 71–78.
25. Namita K., Sonia S. Allium cepa root chromosomal aberration assay: A review. Indian Journal of Pharmacological and Biological Research. 2013; 1(3): 105-119.
26. Ibanrikynti T., Manash P. B., Surya B. P. Inhibitory effect of propolis on onion (Allium cepa) root tip meristem cells. Journal of Science. 2015; 5(12): 1345-1349.
27. Poosala K., Saarangi R., Mahesh V. Wheat rootlet growth inhibition assay and antimitotic studies of some N-1, N-3 bis [6-chlorobenzo (d) thiazol-2-yl] 2–substituted methyl malonamides. Scholars Journal of Applied Medical Sciences. 2013; 1(3): 219-225.
28. Mudeenahally H. K., Basavaiah U., Santhekasalagere B. S. and Yeriyur B. B. Synthesis and study of antimitotic activity of new tetralone esters. International Journal of Chemical and Pharmaceutical Sciences. 2014; 5(3): 105-111.
29. N. S. Patil, K. B. Patil, M. R. Patil, R. A. Ahirrao. Antimitotic Activity of Fruits of Momordica dioica by using Allium cepa Root Tip Assay. Asian J. Pharm. Res. 2018; 8(4): 221-224.
30. S. Selvaraju, M. Vasanth, R. Rajarajan, V. Raghupathy. Cytogenetic Activities of Fungicide on the Root Apicalmeristems of Onion Plant (Allium cepa L.). Asian J. Res. Pharm. Sci. 2014; 4(4): 160-164.
31. S Selvaraju, M Vasanth, R Rajarajan, R Muralidharan, V Raghupathy. Genotoxic Effects of Carbendezim (fungicide) on the Root Apicalmeristems of Allium cepa L. Res. J. Pharmacognosy and Phytochem. 2015; 7(1): 29-33.
32. Himshikha Yadav, Sushil Kumar. An Assessment of Cytotoxic Potentiality of Plantago ovata Husk Aqueous Suspension on the Root Meristem Cells of Vicia faba L. Research J. Pharm. and Tech. 2017; 10(7): 2053-2057.
33. Katolkar P.P., Nimbekar T.P., Wanjari B.E., Nema M.V., Dongarwar A. S. In-vitro antimitotic activity and antiproliferative activity of different extract of Belanites aegyptiaca. Research J. Pharm. and Tech. 2012; 5(4): 474-477.