Detection of Ganja and Nicotine Admixture by a New System


Kamalakshi Krishnamurthy1, Thant Zin2, K. Priyamvatha3, Mahadeva Rao U.S.2*, Suganya M.4

1Additional Director &Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai, India.

2School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA),

Kuala Terengganu, Malaysia.

3Former Student of Women's Christian College, Chennai, India.

4People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India.

*Corresponding Author E-mail:,



Drug abuse has become more prevalent in India. Smokers mix Ganja (marijuana) along with tobacco in the cigarettes or cigars. Necessity has arisen at this juncture to separately locate Ganja in the admixture of Tobacco and Ganja. The present work envisages a new developing as well as identification method for separating Ganja from Tobacco by Thin Layer chromatography simultaneously in the same plate.


KEYWORDS: Marijuana, Tobacco, Drug abuse, cigarettes




Ganja belongs to the family Cannabinaceae. {Cannabaceae} Major Cannabinoids are Cannabinol, Cannabidiol and Tetrahydro Cannabinol. Nicotine belongs to natural order Solanaceae. Nicotiana Tobacum is cultivated largely all over India. Dried leaves are used as snuff, for smoking, chew them with lime or with lime and pan.


The leaves yield 2 active principles namely Nicotine and Nicotianine. Nicotine has a burning acrid taste and a penetrating disagreeable odour. It stimulates and then paralyses the cerebral and spinal centres. Cancer has been reported in patients who used tobacco for long time.



Thin Layer Chromatography is a good, alternative method to some of the reported expensive instrumental methods. The present work envisages a new developing as well as identification method for separating in the Ganja from Tobacco by TLC simultaneously in the same plate.


TLC plate coated with Silica Gel GF 254 used.




1 Propanol:







System allowed to saturate. Control Ganja and Nicotine are spotted each separately. Admixture of Ganja and Nicotine spotted. The plate is run to sufficient height, removed and allowed to dry.


Spray I: 0.1% Fast Blue B followed by 5% Sodium Hydroxide. Spots of Ganja revealed at a Rf 0.8.


Spray II: Dragendorff (equal mixture of 1 and 2) followed by neutral ferric chloride solution. Nicotine revealed at a Rf 0.35 both for individual specimens and for the admixture.



1.      Textbook of Medical Jurisprudence and Toxicology - By Jaising Modi

2.      Forensic Medicine and Toxicology - By V. Dekal.

3.      Text book of Medical Jurisprudence and Toxicology - By C.K. Parikh.

4.      Essentials of Forensic Medicine and Toxicology - By K.S. Narayan Reddy and O.P. Murthy.

5.      Thin - Layer Chromatography - Egon Stahl.

6.      Indian Medicinal Plants - By Kirti and Basuthkar.



Received on 17.05.2022           Modified on 14.06.2022

Accepted on 21.07.2022   ©Asian Pharma Press All Right Reserved

Asian J. Res. Pharm. Sci. 2022; 12(3):194.

DOI: 10.52711/2231-5659.2022.00033