Volume No. :   7

Issue No. :  3

Year :  2017

ISSN Print :  2231-5640

ISSN Online :  2231-5659


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A New Simultaneous HPLC Analytical method for Quantification of Benazepril Hydrochloride and its related Impurities in Bulk Drug Product



Address:   Tentu NageswaraRao1*, Karri Apparao1, N. Krishnarao1, A. Vijayalakshmi2
1Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.
2Department of Marine Living Resources, Andhra University, Visakhapatnam, Andhra Pradesh, India.
*Corresponding Author
DOI No: 10.5958/2231-5659.2017.00022.4

ABSTRACT:
A simple and inexpensive method was developed with high performance liquid chromatography with PDA detection for determination of benazepril hydrochloride and its related impurities. The chromatographic separations were achieved on (250×4.6 mm), 5.0 µm make: Symmetry Shield column employing 0.02M tetrabuthylammonium hydroxide + 0.05 % v/v acetic acid : methanol in the ratio of 50:50 (v/v) as mobile phase with isocratic at flow rate 1.0mL/min was chosen. All impurities were eluted within 30 minutes. The column temperature was maintained at 25oC and a detector wavelength of 240 nm was employed. The method was successfully validated by establishing System Suitability, Specificity, Linearity, Precision, Accuracy, Limit of detection and Limit of quantification.
KEYWORDS:
HPLC, Method validation, related impurities, Benazepril hydrochloride, LOQ, LOD.
Cite:
Tentu NageswaraRao, Karri Apparao, N. Krishnarao, A. Vijayalakshmi. A New Simultaneous HPLC Analytical method for Quantification of Benazepril Hydrochloride and its related Impurities in Bulk Drug Product. Asian J. Res. Pharm. Sci. 2017; 7(3): 135-140.
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