Volume No. :   6

Issue No. :  4

Year :  2016

ISSN Print :  2231-5640

ISSN Online :  2231-5659


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Development of a Validated RP-HPLC Method for Estimation of Ethionamide in Spiked Human Plasma with UV Detection



Address:   Priya Rahade, Sandeep Sonawane*, Atharva Bhalerao, Sanjay Kshirsagar
Department of Pharmaceutical Analysis, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik – 422 003
*Corresponding Author
DOI No:

ABSTRACT:
A simple, rapid and economic RP-HPLC method was developed and validated for estimation of Ethionamide in spiked human plasma. The drug along with Guaifenesin (used as internal standard) was extracted from plasma by liquid-liquid extraction (LLE) procedure using ethyl acetate as organic solvent. The drug was well resolved from the plasma interference and internal standard in a reversed phase mode on C 18 (250 × 4.6 mm, 5 µ) column with methanol: water (40: 60 %, v/v) as mobile phase, at a flow rate of 1 mL/min. The detection was performed at 275 nm. The developed method was validated as per the US-FDA guidelines, where the weighted linear regression was used in calibration experiments to obtain the homoscedasticity. In accuracy and precision studies, intra-day and inter-day, % relative error was found between ± 15 and % RSD was less than 15 %. Stability experiments indicated that the drug remained stable after three freeze-thaw cycles.
KEYWORDS:
Ethionamide, Bioanalytical, Liquid-liquid extraction, Weighted regression, RP-HPLC.
Cite:
Priya Rahade, Sandeep Sonawane, Atharva Bhalerao, Sanjay Kshirsagar. Development of a Validated RP-HPLC Method for Estimation of Ethionamide in Spiked Human Plasma with UV Detection. Asian J. Res. Pharm. Sci. 2016; 6(4): 230-234.
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