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Asian Journal of Research in Pharmaceutical Sciences
ISSN: 2231-5659(Online), 2231-5640(Print)
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First derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in synthetic mixture
Divya A. Patel
Hasumati A. Raj
Vineet C. Jain
Divya A. Patel*, Hasumati A. Raj, Vineet C. Jain
Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, India.
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 351.00 nm while Argatroban showed zero crossing point at 280.47 nm. The dA/d? was measured at 280.47 nm for Edaravone and 351.00nm for Argatroban and calibration curves were plotted as dA/d? versus concentration, respectively. The method was found to be linear (r2>0.998) in the range of 10-35µg/ml for Edaravone at 280.47 nm. The linear correlation was obtained (r2>0.999) in the range of 10-35 µg/ml for Argatroban at 351.0 nm. The limit of determination was 1.59µg/ml and 1.87µg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 4.83 µg/ml and 5.68 µg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.
Edaravone, Argatroban , First Derivative Method, Spectroscopic method.
Divya A. Patel, Hasumati A. Raj, Vineet C. Jain. First derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in synthetic mixture. Asian J. Res. Pharm. Sci. 5(1): Jan.-March 2015; Page 18-26.
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