Volume No. :   5

Issue No. :  1

Year :  2015

ISSN Print :  2231-5640

ISSN Online :  2231-5659


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First derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in synthetic mixture



Address:   Divya A. Patel*, Hasumati A. Raj, Vineet C. Jain
Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, India.
*Corresponding Author
DOI No: 10.5958/2231-5659.2015.00004.1

ABSTRACT:
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 351.00 nm while Argatroban showed zero crossing point at 280.47 nm. The dA/d? was measured at 280.47 nm for Edaravone and 351.00nm for Argatroban and calibration curves were plotted as dA/d? versus concentration, respectively. The method was found to be linear (r2>0.998) in the range of 10-35µg/ml for Edaravone at 280.47 nm. The linear correlation was obtained (r2>0.999) in the range of 10-35 µg/ml for Argatroban at 351.0 nm. The limit of determination was 1.59µg/ml and 1.87µg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 4.83 µg/ml and 5.68 µg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.
KEYWORDS:
Edaravone, Argatroban , First Derivative Method, Spectroscopic method.
Cite:
Divya A. Patel, Hasumati A. Raj, Vineet C. Jain. First derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in synthetic mixture. Asian J. Res. Pharm. Sci. 5(1): Jan.-March 2015; Page 18-26.
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