Volume No. :   4

Issue No. :  4

Year :  2014

ISSN Print :  2231-5640

ISSN Online :  2231-5659


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Spectrophotometric Investigations on Cephalosporines by Ellman’s Reagent in Bulk and Dosage Forms



Address:   Sobhy M. El-Adl, Mohamed El. Hossinny El. Sadek , Marwa Hamdy Hassan
Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt
*Corresponding Author
DOI No:

ABSTRACT:
An quantitative spectrophotometric method for determination of cefotaxime Na and cefixime. The developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with DTNB as a chromogenic reagent which could be used for their analysis in pure forms and in pharmaceutical formulations then measuring absorbances at (417nm for cefotaxime and cefixime at 411 nm). The concentration of NaOH, DTNB concentration and hydrolysis time were studied. Calibration curves were linear over ranges of 0.8–4 µg.ml-1 for Cefotaxime and 0.4- 2 µg.ml-1 for Cefixime. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.
KEYWORDS:
Cefotaxime, Cefixime, NaOH, DTNB.
Cite:
Sobhy M. El-Adl, Mohamed El. Hossinny El. Sadek , Marwa Hamdy Hassan. Spectrophotometric Investigations on Cephalosporines by Ellman’s Reagent in Bulk and Dosage Forms. Asian J. Res. Pharm. Sci. 4(4): Oct.-Dec. 2014; Page 196-201.
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